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PurposeProduction of recombinant Ufd1-His in E.coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 117107 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET41b+
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameUfd1
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SpeciesH. sapiens (human)
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Entrez GeneUFD1 (a.k.a. UFD1L)
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Tag
/ Fusion Protein
- His (C terminal on insert)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET41b+_Ufd1-His was a gift from Hemmo Meyer (Addgene plasmid # 117107 ; http://n2t.net/addgene:117107 ; RRID:Addgene_117107) -
For your References section:
Ubiquitin-Independent Disassembly by a p97 AAA-ATPase Complex Drives PP1 Holoenzyme Formation. Weith M, Seiler J, van den Boom J, Kracht M, Hulsmann J, Primorac I, Del Pino Garcia J, Kaschani F, Kaiser M, Musacchio A, Bollen M, Meyer H. Mol Cell. 2018 Oct 11. pii: S1097-2765(18)30791-3. doi: 10.1016/j.molcel.2018.09.020. 10.1016/j.molcel.2018.09.020 PubMed 30344098