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Addgene

OsMATE
(Plasmid #117093)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 117093 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPICZc
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin (Zeocin), 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AtTPT (ORF-HIS)
  • Alt name
    tr|G9M7W8|G9M7W8_ORYSJ MATE efflux family protein OS=Oryza sativa subsp. japonica GN=OsFRDL4 PE=2 SV=1
  • Promoter AOX
  • Tag / Fusion Protein
    • His (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer GACTGGTTCCAATTGACAAGC
  • 3′ sequencing primer GCAAATGGCATTCTGACATCC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

An A234T difference in AtTPT compared to the reference sequence is present but does not impact the function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    OsMATE was a gift from Geoffrey Chang (Addgene plasmid # 117093 ; http://n2t.net/addgene:117093 ; RRID:Addgene_117093)
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