AtPIN2
(Plasmid
#117089)
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Purposeprotein expression in yeast
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 117089 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepPICZc
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Bleocin (Zeocin), 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAtPIN2
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Alt nameENA|AAC39513|AAC39513.1 Arabidopsis thaliana (thale cress) auxin transport protein EIR1
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SpeciesA. thaliana (mustard weed)
- Promoter AOX
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Tag
/ Fusion Protein
- His (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer GACTGGTTCCAATTGACAAGC
- 3′ sequencing primer GCAAATGGCATTCTGACATCC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AtPIN2 was a gift from Geoffrey Chang (Addgene plasmid # 117089 ; http://n2t.net/addgene:117089 ; RRID:Addgene_117089)