V181 pLPS 3' Triple-Flag - Acceptor
(Plasmid
#11708)
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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 11708 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backboneNA
- Backbone size (bp) 4234
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Modifications to backboneSplice for 3' Tags
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Vector typeMammalian Expression, Cre/Lox ; Acceptor vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- 3xFLAG (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Acceptor vector for the Creator Splice system.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
V181 pLPS 3' Triple-Flag - Acceptor was a gift from Tony Pawson (Addgene plasmid # 11708 ; http://n2t.net/addgene:11708 ; RRID:Addgene_11708) -
For your References section:
Modification of the Creator recombination system for proteomics applications--improved expression by addition of splice sites. Colwill K, Wells CD, Elder K, Goudreault M, Hersi K, Kulkarni S, Hardy WR, Pawson T, Morin GB. BMC Biotechnol. 2006 . 6():13. 10.1186/1472-6750-6-13 PubMed 16519801
Map uploaded by the depositor.
