pSpot2
(Plasmid #117063)

• Purpose: (Empty Backbone) For expression of proteins with C-terminal Spot-Tag in E. coli
• Depositing Lab: Felix Hartlepp
• Publication: ChromoTek Spot-tag plasmids (unpublished)

Backbone

• Vector backbone: pTrc
• Vector type: Bacterial Expression
• Tag / Fusion Protein:
  • Spot-Tag (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme

Resource Information

• Supplemental Documents:
  • pSpot2.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry