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pJL1-mRFP1 gRNA
(Plasmid #117052)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 117052 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJL1 (modified)
  • Backbone size w/o insert (bp) 1631
  • Total vector size (bp) 1777
  • Modifications to backbone
    deleted ribosome binding site, spacer nucleotides between T7 promoter, insert, and terminator
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    anti-mRFP1 SpCas9 gRNA
  • Species
    Synthetic
  • Insert Size (bp)
    146
  • Promoter T7

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJL1-mRFP1 gRNA was a gift from Michael Jewett (Addgene plasmid # 117052 ; http://n2t.net/addgene:117052 ; RRID:Addgene_117052)

  • For your References section:

    BioBits Health: Classroom Activities Exploring Engineering, Biology, and Human Health with Fluorescent Readouts. Stark JC, Huang A, Hsu KJ, Dubner RS, Forbrook J, Marshalla S, Rodriguez F, Washington M, Rybnicky GA, Nguyen PQ, Hasselbacher B, Jabri R, Kamran R, Koralewski V, Wightkin W, Martinez T, Jewett MC. ACS Synth Biol. 2019 May 7. doi: 10.1021/acssynbio.8b00381. 10.1021/acssynbio.8b00381 PubMed 30925042
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