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lenti F9-TetR-EGFP-IRES-PuroR
(Plasmid #117049)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 117049 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Cre-IRES-PuroR
  • Backbone manufacturer
    Addgene Plasmid #30205
  • Backbone size w/o insert (bp) 7177
  • Total vector size (bp) 9451
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Growth in a bacterial strain that is suitable for repetitive sequences is recommended.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    F9-TetR-EGFP
  • Insert Size (bp)
    2274
  • Promoter F9 promoter
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site PshAI (not destroyed)
  • 5′ sequencing primer GAGGCATTGTAGAGGCTTC and AGGTCGACTCTAGACGATC
  • 3′ sequencing primer IRES-R
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    F9-TetR-EGFP sequence was obtained from Dr. Andrew Belmont.
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    lenti F9-TetR-EGFP-IRES-PuroR was a gift from Huimin Zhao (Addgene plasmid # 117049 ; http://n2t.net/addgene:117049 ; RRID:Addgene_117049)

  • For your References section:

    CRISPR/Cas9-mediated knock-in of an optimized TetO repeat for live cell imaging of endogenous loci. Tasan I, Sustackova G, Zhang L, Kim J, Sivaguru M, HamediRad M, Wang Y, Genova J, Ma J, Belmont AS, Zhao H. Nucleic Acids Res. 2018 Jun 15. pii: 5038283. doi: 10.1093/nar/gky501. 10.1093/nar/gky501 PubMed 29912475
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