pT4-CMV-GFP
(Plasmid #117046)

• Purpose: Optimized Sleeping Beauty Transposon Vector for GFP expression by a CMV promoter
• Depositing Lab: Wolfgang Uckert
• Publication: Optimized Sleeping Beauty Transposon Vector with CMV-GFP cassette (unpublished)

Backbone

• Vector backbone: pBluescript
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3457
• Total vector size (bp): 4999
• Modifications to backbone: Sleeping Beauty terminal inverted repeats inserted which are engulfing a CMV-GFP transgene cassette.
• Vector type: Transposon

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GFP
• Insert Size (bp): 720
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: GFP-FWD
• 3′ sequencing primer: GFP-REV

Resource Information

• Supplemental Documents:
  • pT4-CMV-GFP.gb
• A portion of this plasmid was derived from a plasmid made by: Julian Clauß, Max-Delbrück-Center for Molecular Medicine, Berlin, Germany
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The Sleeping Beauty transposon system consists of two components: the transposon vector and the transposase vector. The transposon vector (empty alternative: pT4/HB, Addgene #108352) contains binding sites flanking the GOI. And the transposase vector (pCMV(CAT)T7-SB100, Addgene #34879) encodes for the enzyme mediating excision and integration of the GOI.