SG-C-Int-14
(Plasmid
#117043)
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PurposeConstitutive integrase cassette - Tp901-Int5-Int7
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 117043 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneJ66100
- Backbone size w/o insert (bp) 2327
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameConstitutive integrase cassette - Tp901-Int5-Int7
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SpeciesSynthetic
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Insert Size (bp)5583
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that some discrepancies were found between Addgene's quality control result and the depositor's provided Genbank sequence. The depositor noted that these discrepancies do NOT affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SG-C-Int-14 was a gift from Jerome Bonnet (Addgene plasmid # 117043 ; http://n2t.net/addgene:117043 ; RRID:Addgene_117043) -
For your References section:
Hierarchical composition of reliable recombinase logic devices. Guiziou S, Mayonove P, Bonnet J. Nat Commun. 2019 Jan 28;10(1):456. doi: 10.1038/s41467-019-08391-y. 10.1038/s41467-019-08391-y PubMed 30692530