pMRXIP-Myc-YKT6
(Plasmid #116945)

• Purpose: Expressing Myc-YKT6 for mammalian cells
• Depositing Lab: Noboru Mizushima
• Publication: Matsui et al J Cell Biol. 2018 Aug 6;217(8):2633-2645. doi: 10.1083/jcb.201712058. Epub 2018 May 22.

Backbone

• Vector backbone: pMRXIP 3xMyc-Ci2
• Backbone manufacturer: Dr.Shoji Yamaoka of Tokyo Medical and Dental University
• Backbone size w/o insert (bp): 6257
• Total vector size (bp): 6854
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Synaptobrevin homolog YKT6
• Alt name: YKT6
• Species: H. sapiens (human)
• Insert Size (bp): 597
• GenBank ID: NM_006555.3 NM_006555.3
• Entrez Gene: YKT6
• Promoter: CMV
• Tag / Fusion Protein:
  • Myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: none
• 3′ sequencing primer: none

Resource Information

• A portion of this plasmid was derived from a plasmid made by: It has the pMX backbone
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry