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pMRXIP-Myc-SNAP29
(Plasmid #116944)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 116944 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMRXIP 3xMyc-Ci2
  • Backbone manufacturer
    Dr.Shoji Yamaoka of Tokyo Medical and Dental University
  • Backbone size w/o insert (bp) 6231
  • Total vector size (bp) 7009
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    synaptosomal-associated protein, 29kDa
  • Alt name
    SNAP29
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    774
  • GenBank ID
    NM_004782.3
  • Entrez Gene
    SNAP29 (a.k.a. CEDNIK, SNAP-29)
  • Promoter CMV
  • Tag / Fusion Protein
    • Myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer none
  • 3′ sequencing primer none
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    It has the pMX backbone

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMRXIP-Myc-SNAP29 was a gift from Noboru Mizushima (Addgene plasmid # 116944 ; http://n2t.net/addgene:116944 ; RRID:Addgene_116944)

  • For your References section:

    Autophagosomal YKT6 is required for fusion with lysosomes independently of syntaxin 17. Matsui T, Jiang P, Nakano S, Sakamaki Y, Yamamoto H, Mizushima N. J Cell Biol. 2018 Aug 6;217(8):2633-2645. doi: 10.1083/jcb.201712058. Epub 2018 May 22. 10.1083/jcb.201712058 PubMed 29789439
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