pLVX puro TRIM21-GFP
(Plasmid #116941)

• Purpose: Expression of TRIM21-GFP fusion protein
• Depositing Lab: Gaudenz Danuser
• Publication: Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586-020-1998-1. doi: 10.1038/s41586-020-1998-1.

Backbone

• Vector backbone: pLVX-puro
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 8140
• Total vector size (bp): 10200
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TRIM21 - GFP
• Species: H. sapiens (human)
• Insert Size (bp): 2148
• Entrez Gene: TRIM21 (a.k.a. RNF81, RO52, Ro/SSA, SSA, SSA1)
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SnaB1 (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: IRES reverse

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry