pRS306-URA3-PHIS3-AqLumSynth –Sapphire
(Plasmid #116931)

• Purpose: Yeast integrative vector for the exression of the 20nm GEM under the HIS3 promoter
• Depositing Lab: Liam Holt
• Publication: Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. doi: 10.1016/j.cell.2018.05.042. Epub 2018 Jun 21.

Backbone

• Vector backbone: pRS306
• Backbone size w/o insert (bp): 4801
• Total vector size (bp): 6040
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: lumazine synthase
• Species: Aquifex aeolicus
• Insert Size (bp): 462
• Promoter: HIS3
• Tag / Fusion Protein:
  • Sapphire (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: M13-fwd
• 3′ sequencing primer: M13-rev

Resource Information

• Supplemental Documents:
  • pRS306-pHIS-Aq_LumSyn-Sapphire.ape
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry