pBSPPc-Gm
(Plasmid
#116919)
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Purpose(Empty Backbone) Gene constitutive expression from a Pc promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 116919 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBSPIISK(-)
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Vector typeBacterial Expression
- Promoter Pc promoter from Delftia acidovorans C17
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The ampicillin resistance gene of the pBSPPc vector (Addgene plasmid # 116918) was replaced with the gentamicin resistance gene from the vector pUC18-mini-Tn7T-Gm.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBSPPc-Gm was a gift from Cuiqing Ma (Addgene plasmid # 116919 ; http://n2t.net/addgene:116919 ; RRID:Addgene_116919) -
For your References section:
New constitutive vectors: useful genetic engineering tools for biocatalysis. Xu Y, Tao F, Ma C, Xu P. Appl Environ Microbiol. 2013 Apr;79(8):2836-40. doi: 10.1128/AEM.03746-12. Epub 2013 Feb 15. 10.1128/AEM.03746-12 PubMed 23416993
