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Addgene

pFGL1344
(Plasmid #116902)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 116902 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFGL1269R
  • Backbone manufacturer
    Naweed Lab
  • Backbone size w/o insert (bp) 8096
  • Total vector size (bp) 10046
  • Modifications to backbone
    Alp6:Bar:mCherry
  • Vector type
    Fungal expression (in Magnaporthe oryzae)
  • Selectable markers
    Basta

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Alp6 (partial ORF)
  • Species
    Magnaporthe oryzae
  • Insert Size (bp)
    1015

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR I (not destroyed)
  • 3′ cloning site Kpn I (not destroyed)
  • 5′ sequencing primer CTCGAATTCGCAATATGCAAGCCCAGAAGTGC
  • 3′ sequencing primer AACGGTACCGTCTCCAGCACGCTCGCCCCTG
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Alp6 downstream region
  • Insert Size (bp)
    982

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xba I (not destroyed)
  • 3′ cloning site Hind III (not destroyed)
  • 5′ sequencing primer TGCTCTAGATAAGTAGTTTGAGAAGGAATGACGC
  • 3′ sequencing primer ATGAAGCTTGGGTCGATGAGTGGTTGGGTT
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFGL1344 was a gift from Naweed Naqvi (Addgene plasmid # 116902 ; http://n2t.net/addgene:116902 ; RRID:Addgene_116902)

  • For your References section:

    Cellular Dynamics and Genomic Identity of Centromeres in Cereal Blast Fungus. Yadav V, Yang F, Reza MH, Liu S, Valent B, Sanyal K, Naqvi NI. MBio. 2019 Jul 30;10(4). pii: mBio.01581-19. doi: 10.1128/mBio.01581-19. 10.1128/mBio.01581-19 PubMed 31363034
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