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pFGL1169R
(Plasmid #116899)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 116899 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFGL822
  • Backbone manufacturer
    Naweed Lab
  • Backbone size w/o insert (bp) 7779
  • Total vector size (bp) 9989
  • Modifications to backbone
    insertion with (truncated) CenpC-mCherry
  • Vector type
    Fungal expression (in Magnaporthe oryzae)
  • Selectable markers
    Basta

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Cenp C (partial)
  • Species
    Magnaporthe oryzae
  • Insert Size (bp)
    562
  • Mutation
    truncated, no stop codon

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR I (not destroyed)
  • 3′ cloning site Kpn I (not destroyed)
  • 5′ sequencing primer CCGGAATTCGAGGACGAAGAACC
  • 3′ sequencing primer GATGGTACCGCTGCTTTCAGTCATTTCGTCC
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    mCherry
  • Species
    Synthetic
  • Insert Size (bp)
    720
  • Mutation
    without start codon

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site Kpn I (not destroyed)
  • 3′ cloning site BamH I (not destroyed)
  • 5′ sequencing primer CTCGGTACCGTGAGCAAGGGCGAGGAGGATAA
  • 3′ sequencing primer CGCGGATCCTTACTTGTACAGCTCGTCCATGC
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    CenpC downstream region
  • Species
    Magnaporthe oryzea
  • Insert Size (bp)
    981

Cloning Information for Gene/Insert 3

  • Cloning method Restriction Enzyme
  • 5′ cloning site Pst I (not destroyed)
  • 3′ cloning site Hind III (not destroyed)
  • 5′ sequencing primer CTGCTGCAGTAATTCATCGCGGTGGGTTGGTC
  • 3′ sequencing primer TGTAAGCTTCTGGCCCTCCCTCATTAT
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFGL1169R was a gift from Naweed Naqvi (Addgene plasmid # 116899 ; http://n2t.net/addgene:116899 ; RRID:Addgene_116899)

  • For your References section:

    Cellular Dynamics and Genomic Identity of Centromeres in Cereal Blast Fungus. Yadav V, Yang F, Reza MH, Liu S, Valent B, Sanyal K, Naqvi NI. MBio. 2019 Jul 30;10(4). pii: mBio.01581-19. doi: 10.1128/mBio.01581-19. 10.1128/mBio.01581-19 PubMed 31363034
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