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pFGL1258
(Plasmid #116898)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 116898 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFGL125_TetGFP(Hyg)
  • Backbone manufacturer
    Naweed Lab
  • Backbone size w/o insert (bp) 11398
  • Total vector size (bp) 13113
  • Modifications to backbone
    CenpA was introduced.
  • Vector type
    Fungal expression (in Magnaporthe oryzae)
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    CenpA promoter region
  • Species
    Magnaporthe oryzae
  • Insert Size (bp)
    809

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR I (not destroyed)
  • 3′ cloning site EcoR I (not destroyed)
  • 5′ sequencing primer ggtGAATTCagtcgcaggtacatctcatta
  • 3′ sequencing primer gtgGAATTCtttatgtcggtttctatatggtttc
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    CenpA
  • Species
    Magnaporthe oryzae
  • Insert Size (bp)
    930

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH I (not destroyed)
  • 3′ cloning site Xba I (not destroyed)
  • 5′ sequencing primer ATAggatccCCACCACAAAAAGTAAAGAAGG
  • 3′ sequencing primer gttTCTAGAaaagcagtccccagagtaactt
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFGL1258 was a gift from Naweed Naqvi (Addgene plasmid # 116898 ; http://n2t.net/addgene:116898 ; RRID:Addgene_116898)

  • For your References section:

    Cellular Dynamics and Genomic Identity of Centromeres in Cereal Blast Fungus. Yadav V, Yang F, Reza MH, Liu S, Valent B, Sanyal K, Naqvi NI. MBio. 2019 Jul 30;10(4). pii: mBio.01581-19. doi: 10.1128/mBio.01581-19. 10.1128/mBio.01581-19 PubMed 31363034
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