pFGL1258
(Plasmid #116898)

• Purpose: TetOFF:GFP:CenpA (centromere marker)
• Depositing Lab: Naweed Naqvi
• Publication: Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581-19. doi: 10.1128/mBio.01581-19.

Backbone

• Vector backbone: pFGL125_TetGFP(Hyg)
• Backbone manufacturer: Naweed Lab
• Backbone size w/o insert (bp): 11398
• Total vector size (bp): 13113
• Modifications to backbone: CenpA was introduced.
• Vector type: Fungal expression (in Magnaporthe oryzae)
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: CenpA promoter region
• Species: Magnaporthe oryzae
• Insert Size (bp): 809

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR I (not destroyed)
• 3′ cloning site: EcoR I (not destroyed)
• 5′ sequencing primer: ggtGAATTCagtcgcaggtacatctcatta
• 3′ sequencing primer: gtgGAATTCtttatgtcggtttctatatggtttc

Gene/Insert 2

• Gene/Insert name: CenpA
• Species: Magnaporthe oryzae
• Insert Size (bp): 930

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH I (not destroyed)
• 3′ cloning site: Xba I (not destroyed)
• 5′ sequencing primer: ATAggatccCCACCACAAAAAGTAAAGAAGG
• 3′ sequencing primer: gttTCTAGAaaagcagtccccagagtaactt

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry