pFGL1170R
(Plasmid #116896)

• Purpose: hH1:mCherry (nuclear marker)
• Depositing Lab: Naweed Naqvi
• Publication: Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581-19. doi: 10.1128/mBio.01581-19.

Backbone

• Vector backbone: pFGL822
• Backbone manufacturer: Naweed Lab
• Backbone size w/o insert (bp): 7779
• Total vector size (bp): 10381
• Modifications to backbone: insertion with hH1:mCherry
• Vector type: Fungal expression (in Magnaporthe oryzae)
• Selectable markers: Basta

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: hH1
• Species: Magnaporthe oryzae
• Insert Size (bp): 1048
• Mutation: no start codon, no stop codon
• Promoter: promoter less

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xho I (not destroyed)
• 3′ cloning site: EcoR1 (not destroyed)
• 5′ sequencing primer: CGTCTCGAGCCTCCCAAGAAGGAAACC
• 3′ sequencing primer: GTCGAATTCTGCGGCGGGTGCCTCGGC

Gene/Insert 2

• Gene/Insert name: mCherry
• Species: Synthetic
• Insert Size (bp): 720
• Mutation: without start codon
• Promoter: promoter less

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: Kpn I (not destroyed)
• 3′ cloning site: BamH I (not destroyed)
• 5′ sequencing primer: CTCGGTACCGTGAGCAAGGGCGAGGAGGATAA
• 3′ sequencing primer: CGCGGATCCTTACTTGTACAGCTCGTCCATGC

Gene/Insert 3

• Gene/Insert name: hH1 downstream region
• Species: Magnaporthe oryzea
• Insert Size (bp): 880

Cloning Information for Gene/Insert 3

• Cloning method: Restriction Enzyme
• 5′ cloning site: Pst I (not destroyed)
• 3′ cloning site: Hind III (not destroyed)
• 5′ sequencing primer: CCGCTGCAGTAAAGGGACGCTGACGAACTT
• 3′ sequencing primer: CATAAGCTTCTTTCTTTGACGGGAAAGGGA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry