pTW051d2
(Plasmid
#116893)
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Purposehigh stringency SE-PACE AP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 116893 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneColE1
- Total vector size (bp) 6540
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin and Streptomycin, 50 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB turbo
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameg3c
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SpeciesSynthetic
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Insert Size (bp)1362
Gene/Insert 2
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Gene/Insert nameT7 RNAP C-term R632S/Q649S
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SpeciesSynthetic
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Insert Size (bp)2118
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTW051d2 was a gift from David Liu (Addgene plasmid # 116893 ; http://n2t.net/addgene:116893 ; RRID:Addgene_116893) -
For your References section:
Continuous directed evolution of proteins with improved soluble expression. Wang T, Badran AH, Huang TP, Liu DR. Nat Chem Biol. 2018 Aug 20. pii: 10.1038/s41589-018-0121-5. doi: 10.1038/s41589-018-0121-5. 10.1038/s41589-018-0121-5 [pii] PubMed 30127387