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Addgene

pTW051d
(Plasmid #116892)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 116892 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    ColE1
  • Total vector size (bp) 6540

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin and Streptomycin, 50 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB turbo
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    g3c
  • Species
    Synthetic
  • Insert Size (bp)
    1362

Gene/Insert 2

  • Gene/Insert name
    T7 RNAP C-term R632S/Q649S
  • Species
    Synthetic
  • Insert Size (bp)
    2118

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTW051d was a gift from David Liu (Addgene plasmid # 116892 ; http://n2t.net/addgene:116892 ; RRID:Addgene_116892)

  • For your References section:

    Continuous directed evolution of proteins with improved soluble expression. Wang T, Badran AH, Huang TP, Liu DR. Nat Chem Biol. 2018 Aug 20. pii: 10.1038/s41589-018-0121-5. doi: 10.1038/s41589-018-0121-5. 10.1038/s41589-018-0121-5 [pii] PubMed 30127387
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