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Addgene

Gag-delta-p6-mEGFP
(Plasmid #116890)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 116890 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Modifications to backbone
    introduced mutation to make EGFP monomeric: A206K
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    synthetic Gag missing the p6 domain
  • Species
    HIV-1
  • Promoter CMV
  • Tag / Fusion Protein
    • mEGFP

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Gag-delta-p6-mEGFP was a gift from Sanford Simon (Addgene plasmid # 116890 ; http://n2t.net/addgene:116890 ; RRID:Addgene_116890)

  • For your References section:

    Timing of ESCRT-III protein recruitment and membrane scission during HIV-1 assembly. Johnson DS, Bleck M, Simon SM. Elife. 2018 Jul 4;7. pii: 36221. doi: 10.7554/eLife.36221. 36221 [pii] PubMed 29972351
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