HA-DN-14-3-3 zeta (R56A/R60A)
(Plasmid #116889)

• Purpose: Expresses HA-tagged human dominant-negative 14-3-3 zeta (R56A/R60A) in mammalian cells
• Depositing Labs: FENG-QIAN Li, Ken-Ichi Takemaru
• Publication: Li et al J Cell Biol. 2008 Jun 30;181(7):1141-54. doi: 10.1083/jcb.200709091. Epub 2008 Jun 23.

Backbone

• Vector backbone: pCS2+HA
• Backbone size w/o insert (bp): 4100
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DN-14-3-3 zeta (R56A/R60A)
• Species: H. sapiens (human)
• Entrez Gene: YWHAZ (a.k.a. 14-3-3-zeta, HEL-S-3, HEL-S-93, HEL4, KCIP-1, POPCHAS, YWHAD)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CCCAAGCTTGATTTAG (SP6)
• 3′ sequencing primer: TTATGCTGAGTGATATC (T7)

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry