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Addgene

HA-14-3-3 eta
(Plasmid #116887)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 116887 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+HA
  • Backbone size w/o insert (bp) 4100
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    14-3-3 eta
  • Species
    H. sapiens (human)
  • Entrez Gene
    YWHAH (a.k.a. YWHA1)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CCCAAGCTTGATTTAG (SP6)
  • 3′ sequencing primer TTATGCTGAGTGATATC (T7)
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-14-3-3 eta was a gift from FENG-QIAN Li & Ken-Ichi Takemaru (Addgene plasmid # 116887 ; http://n2t.net/addgene:116887 ; RRID:Addgene_116887)

  • For your References section:

    Chibby cooperates with 14-3-3 to regulate beta-catenin subcellular distribution and signaling activity. Li FQ, Mofunanya A, Harris K, Takemaru K. J Cell Biol. 2008 Jun 30;181(7):1141-54. doi: 10.1083/jcb.200709091. Epub 2008 Jun 23. 10.1083/jcb.200709091 PubMed 18573912
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