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PurposeExpresses HA-tagged human 14-3-3 epsilon in mammalian cells
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 116886 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCS2+HA
- Backbone size w/o insert (bp) 4100
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name14-3-3 epsilon
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SpeciesH. sapiens (human)
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Entrez GeneYWHAE (a.k.a. 14-3-3E, HEL2, KCIP-1, MDCR, MDS)
- Promoter CMV
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Tag
/ Fusion Protein
- HA (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer CCCAAGCTTGATTTAG (SP6)
- 3′ sequencing primer TTATGCTGAGTGATATC (T7) (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HA-14-3-3 epsilon was a gift from FENG-QIAN Li & Ken-Ichi Takemaru (Addgene plasmid # 116886 ; http://n2t.net/addgene:116886 ; RRID:Addgene_116886) -
For your References section:
Chibby cooperates with 14-3-3 to regulate beta-catenin subcellular distribution and signaling activity. Li FQ, Mofunanya A, Harris K, Takemaru K. J Cell Biol. 2008 Jun 30;181(7):1141-54. doi: 10.1083/jcb.200709091. Epub 2008 Jun 23. 10.1083/jcb.200709091 PubMed 18573912
