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Addgene

pNIA-CEN-FLAG-LINXa4(RVH)-Bre1
(Plasmid #116885)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 116885 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNIA-CEN
  • Backbone size w/o insert (bp) 8084
  • Total vector size (bp) 10700
  • Vector type
    Yeast Expression, Synthetic Biology
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LINXa4(RVH)-Bre1
  • Species
    S. cerevisiae (budding yeast), Synthetic; Avena sativa
  • Mutation
    Bre1 point mutations K8A, K9A and K11A, LINXa3 wild type HVR 519-521 to RVH
  • Promoter ADH1
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer GTCATTGTTCTCGTTCCCTTTCTTCCTTG
  • 3′ sequencing primer GGGACCTAGACTTCAGGTTGTCTAACTCC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNIA-CEN-FLAG-LINXa4(RVH)-Bre1 was a gift from Brian Kuhlman (Addgene plasmid # 116885 ; http://n2t.net/addgene:116885 ; RRID:Addgene_116885)

  • For your References section:

    Engineering Improved Photoswitches for the Control of Nucleocytoplasmic Distribution. Lerner AM, Yumerefendi H, Goudy OJ, Strahl BD, Kuhlman B. ACS Synth Biol. 2018 Nov 29. doi: 10.1021/acssynbio.8b00368. 10.1021/acssynbio.8b00368 PubMed 30441907
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