pNIA-CEN-FLAG-LINXa4(RVH)-Bre1
(Plasmid #116885)

• Purpose: FLAG-tagged LINXa4-Bre1 with RVH hinge-loop mutations
• Depositing Lab: Brian Kuhlman
• Publication: Lerner et al ACS Synth Biol. 2018 Nov 29. doi: 10.1021/acssynbio.8b00368.

Backbone

• Vector backbone: pNIA-CEN
• Backbone size w/o insert (bp): 8084
• Total vector size (bp): 10700
• Vector type: Yeast Expression, Synthetic Biology
• Selectable markers: LEU2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LINXa4(RVH)-Bre1
• Species: S. cerevisiae (budding yeast), Synthetic; Avena sativa
• Mutation: Bre1 point mutations K8A, K9A and K11A, LINXa3 wild type HVR 519-521 to RVH
• Promoter: ADH1
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: GTCATTGTTCTCGTTCCCTTTCTTCCTTG
• 3′ sequencing primer: GGGACCTAGACTTCAGGTTGTCTAACTCC

Resource Information

• Supplemental Documents:
  • pNIA-CEN-FLAG-LINXa4(RVH)-Bre1.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry