NES-EGFP-PH-ARNO2G-I303Ex2
(Plasmid #116868)

• Purpose: PIP3 biosensor
• Depositing Lab: Gerry Hammond
• Publication: Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079. doi: 10.1083/jcb.201809026. Epub 2018 Dec 27.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Total vector size (bp): 6291
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CYTH2
• Species: H. sapiens (human)
• Insert Size (bp): 447
• Mutation: amind acids 252-399, isoleucine 303 changed to glutamic acid; tandem dimer
• Entrez Gene: CYTH2 (a.k.a. ARNO, CTS18, CTS18.1, PSCD2, PSCD2L, SEC7L, Sec7p-L, Sec7p-like, cytohesin-2)
• Tags / Fusion Proteins:
  • EGFP (N terminal on insert)
  • X.leavis map2k1.L(32-44) (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: EGFP-C1
• 3′ sequencing primer: SV40-pArev

Resource Information

• Supplemental Documents:
  • pNES-EGFP-C1-PHx2-ARNO-2G-I303E.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/early/2018/09/06/410811 for bioRxiv preprint.