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NES-EGFP-PH-ARNO2G-I303Ex2
(Plasmid #116868)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 116868 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Total vector size (bp) 6291
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CYTH2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    447
  • Mutation
    amind acids 252-399, isoleucine 303 changed to glutamic acid; tandem dimer
  • Entrez Gene
    CYTH2 (a.k.a. ARNO, CTS18, CTS18.1, PSCD2, PSCD2L, SEC7L, Sec7p-L, Sec7p-like, cytohesin-2)
  • Tags / Fusion Proteins
    • EGFP (N terminal on insert)
    • X.leavis map2k1.L(32-44) (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/early/2018/09/06/410811 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NES-EGFP-PH-ARNO2G-I303Ex2 was a gift from Gerry Hammond (Addgene plasmid # 116868 ; http://n2t.net/addgene:116868 ; RRID:Addgene_116868)

  • For your References section:

    A high-avidity biosensor reveals plasma membrane PI(3,4)P2 is predominantly a class I PI3K signaling product. Goulden BD, Pacheco J, Dull A, Zewe JP, Deiters A, Hammond GRV. J Cell Biol. 2019 Mar 4;218(3):1066-1079. doi: 10.1083/jcb.201809026. Epub 2018 Dec 27. 10.1083/jcb.201809026 PubMed 30591513
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