pNES-iRFP-C1
(Plasmid #116863)

• Purpose: cytosolic iRFP
• Depositing Lab: Gerry Hammond
• Publication: Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079. doi: 10.1083/jcb.201809026. Epub 2018 Dec 27.

Backbone

• Vector backbone: piRFP-C1
• Total vector size (bp): 5007
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: iRFP713
• Alt name: iRFP
• Species: Rhodopseudomonas palustris
• Insert Size (bp): 948
• Tag / Fusion Protein:
  • X.leavis map2k1.L(32-44) (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: CMV-For
• 3′ sequencing primer: SV40-pArev

Resource Information

• Supplemental Documents:
  • pNES-iRFP-C1.gb
• A portion of this plasmid was derived from a plasmid made by: Addgene 54786
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/early/2018/09/06/410811 for bioRxiv preprint.