NES-PAmCherry-cPHx2
(Plasmid #116860)

• Purpose: PI(3,4)P2 biosensor
• Depositing Lab: Gerry Hammond
• Publication: Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079. doi: 10.1083/jcb.201809026. Epub 2018 Dec 27.

Backbone

• Vector backbone: pPAmCherry-C1
• Total vector size (bp): 5706
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PLEKHA1
• Species: H. sapiens (human)
• Insert Size (bp): 483
• Mutation: amino acids 169-329 (tandem dimer)
• Entrez Gene: PLEKHA1 (a.k.a. TAPP1)
• Tag / Fusion Protein:
  • PAmCherry (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: CMV-For
• 3′ sequencing primer: SV40-pArev

Resource Information

• Supplemental Documents:
  • pPamCherry1-C1-TAPP1-cPHx2.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/early/2018/09/06/410811 for bioRxiv preprint.