Skip to main content
Addgene

pL3-TRE-LucGFP-2L
(Plasmid #11685)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11685 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pL3-TRE-MCS-polyA-2L
  • Backbone manufacturer
    Wahl lab
  • Backbone size w/o insert (bp) 7117
  • Vector type
    Mammalian Expression, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Luciferase-EGFP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI/BglII (destroyed during cloning)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer See map
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    LucGFP fragment was cloned from pLuciferase-EGFP vector (gift of D. Buscher).
  • Article Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pL3-TRE-LucGFP-2L was a gift from Geoff Wahl (Addgene plasmid # 11685 ; http://n2t.net/addgene:11685 ; RRID:Addgene_11685)

  • For your References section:

    Reproducible doxycycline-inducible transgene expression at specific loci generated by Cre-recombinase mediated cassette exchange. Wong ET, Kolman JL, Li YC, Mesner LD, Hillen W, Berens C, Wahl GM. Nucleic Acids Res. 2005 . 33(17):e147. 10.1093/nar/gni145 PubMed 16204450
Related items:
Commonly requested with: