RV-L3-HyTK-2L
(Plasmid #11684)

• Purpose: (Empty Backbone)
• Depositing Lab: Geoff Wahl
• Publication: Wong et al Nucleic Acids Res. 2005 . 33(17):e147.

Backbone

• Vector backbone: pCFB-EGSH
• Backbone manufacturer: Stratagene
• Backbone size (bp): 9177
• Modifications to backbone: new loxP site L3 (see paper)
• Vector type: Mammalian Expression, Retroviral, Cre/Lox
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: See map

Resource Information

• A portion of this plasmid was derived from a plasmid made by: H1HyTK2L cassette was from S. Fiering. It was modified to become L3HyTK2L.
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

L3HyTK2L was constructed by replacing L1 in L1HyTK2L with L3. This cassette was cloned into the backbone by inserting L3HyTK2L restrcted with NotI and XbaI into pCFB-EGSH digested with the same enzymes.