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Addgene

H2B-GFP Notes

Addgene diagnostic digest for 11680

This gel depicts the NEB 1kb ladder and a digestion of the plasmid with the following enzyme(s): BamHI-HF, XhoI, and BamHI-HF/XhoI.

We expected the plasmid to be linearized by digestion with either BamHI-HF or XhoI giving a band size of ~5100bp. A double digest with BamHI-HF and XhoI should result in the following band sizes: 4.7kb and 0.4kb. We see the expected pattern.