GFP-CDC12(WT)
(Plasmid
#1168)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 1168 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRS316
- Backbone size w/o insert (bp) 4895
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCDC12
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)708
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MutationAdded N-terminal GFP to CDC12 in plasmid CDC12(WT)... see article for cloning details
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GenBank IDX82498
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Entrez GeneCDC12 (a.k.a. YHR107C, CLA10, PSL7)
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Tag
/ Fusion Protein
- GFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Eco47III (destroyed during cloning)
- 3′ cloning site Eco47III (destroyed during cloning)
- 5′ sequencing primer n/a
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
7086
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GFP-CDC12(WT) was a gift from Susan Lindquist (Addgene plasmid # 1168 ; http://n2t.net/addgene:1168 ; RRID:Addgene_1168) -
For your References section:
Dominant gain-of-function mutations in Hsp104p reveal crucial roles for the middle region. Schirmer EC, Homann OR, Kowal AS, Lindquist S. Mol Biol Cell 2004 May;15(5):2061-72. Epub 2004 Feb 20. 10.1091/mbc.e02-08-0502 PubMed 14978213
