pPRIME-TET-GIN-recipient
(Plasmid #11656)

• Purpose: (Empty Backbone) 3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with a tetracycline-responsive promoter (TET) controlling GFP-IRES-Neo expression
• Depositing Lab: Stephen Elledge
• Publication: Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 102(37):13212-7.

Backbone

• Vector backbone: pPRIME-TET-GIN-recipient
• Backbone size (bp): 9747
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: None
• Tags / Fusion Proteins:
  • GFP (N terminal on backbone)
  • IRES (N terminal on backbone)
  • Neo (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: EGFP-C

Resource Information

• Supplemental Documents:
  • Elledge-pPRIME

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This recipient plasmid contains pheS Gly294 between the I-SceI sites, which can be selected against using Cl-Phe.