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Depositing Labs
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 11653 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneNone
- Backbone size w/o insert (bp) 9931
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Vector typeMammalian Expression, Lentiviral, RNAi, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Growth instructionsUse Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGFP, shRNA against TP53
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Alt nameshRNA against p53
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Alt namep53
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SpeciesH. sapiens (human)
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Entrez GeneTP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer See map (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit the Trono lab website: http://tronolab.epfl.ch to see frequently asked questions on cloning strategies and packaging.
You may also visit LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.
shRNA sequence 5'-AGTAGATTACCACTGGAGTCTT
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLVUHshp53 was a gift from Patrick Aebischer & Didier Trono (Addgene plasmid # 11653 ; http://n2t.net/addgene:11653 ; RRID:Addgene_11653) -
For your References section:
A versatile tool for conditional gene expression and knockdown. Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P. Nat Methods. 2006 Feb . 3(2):109-16. 10.1038/nmeth846 PubMed 16432520