pGEX4T moesin 307-577
(Plasmid #11639)

• Depositing Lab: Vijaya Ramesh
• Publication: Gonzalez-Agosti et al J Biol Chem. 1999 Nov 26. 274(48):34438-42.

Backbone

• Vector backbone: pGEX4T1
• Backbone manufacturer: Amersham
• Backbone size w/o insert (bp): 4970
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: moesin
• Species: H. sapiens (human)
• Insert Size (bp): 813
• Mutation: Carboxy-terminal half of moesin.
• Entrez Gene: MSN (a.k.a. HEL70, IMD50)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: pGEX5'

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry