pcDNA3 NHE-RF
(Plasmid #11635)

• Depositing Lab: Vijaya Ramesh
• Publication: Gonzalez-Agosti et al J Biol Chem. 1999 Nov 26. 274(48):34438-42.

Backbone

• Vector backbone: pcDNA3/5'F
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NHE-RF
• Alt name: regulatory cofactor of Na-H exchanger
• Alt name: NHERF1
• Species: H. sapiens (human)
• Insert Size (bp): 1014
• Entrez Gene: NHERF1 (a.k.a. EBP50, NHE-RF, NHERF, NHERF-1, NPHLOP2, SLC9A3R1)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry