pGEX2T merlin 340-590
(Plasmid
#11633)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 11633 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX2T
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Backbone manufacturerAmersham
- Backbone size w/o insert (bp) 4950
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemerlin
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Alt nameNF2
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SpeciesH. sapiens (human)
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Insert Size (bp)753
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MutationCarboxy-terminal half of merlin isoform 2.
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Entrez GeneNF2 (a.k.a. ACN, BANF, SCH, SWNV, merlin-1)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pGEX5' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX2T merlin 340-590 was a gift from Vijaya Ramesh (Addgene plasmid # 11633 ; http://n2t.net/addgene:11633 ; RRID:Addgene_11633) -
For your References section:
Interdomain interaction of merlin isoforms and its influence on intermolecular binding to NHE-RF. Gonzalez-Agosti C, Wiederhold T, Herndon ME, Gusella J, Ramesh V. J Biol Chem. 1999 Nov 26. 274(48):34438-42. 10.1074/jbc.274.48.34438 PubMed 10567424