pcDNA3 merlin 308-579
(Plasmid #11626)

• Depositing Lab: Vijaya Ramesh
• Publication: Xu et al Exp Cell Res. 1998 Jan 10. 238(1):231-40.

Backbone

• Vector backbone: pcDNA3/5'F
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: merlin
• Alt name: NF2
• Species: H. sapiens (human)
• Insert Size (bp): 816
• Mutation: Carboxy-terminal common domain of merlin.
• Entrez Gene: NF2 (a.k.a. ACN, BANF, SCH, SWNV, merlin-1)
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry