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Addgene

pcDNA3 merlin 1-332
(Plasmid #11625)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11625 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3/5'F
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    merlin
  • Alt name
    NF2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    996
  • Mutation
    Amino-terminal half of merlin.
  • Entrez Gene
    NF2 (a.k.a. ACN, BANF, SCH, SWNV, merlin-1)
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Named pcDNA3/5'F-N21 (aa 1-332) in reference.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 merlin 1-332 was a gift from Vijaya Ramesh (Addgene plasmid # 11625 ; http://n2t.net/addgene:11625 ; RRID:Addgene_11625)

  • For your References section:

    Analysis of molecular domains of epitope-tagged merlin isoforms in Cos-7 cells and primary rat Schwann cells. Xu L, Gonzalez-Agosti C, Beauchamp R, Pinney D, Sterner C, Ramesh V. Exp Cell Res. 1998 Jan 10. 238(1):231-40. 10.1006/excr.1997.3843 PubMed 9457076
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