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Addgene

pLB-915
(Plasmid #11620)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11620 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLB
  • Backbone manufacturer
    N/A
  • Backbone size w/o insert (bp) 8500
  • Vector type
    Mammalian Expression, Lentiviral, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Slc11a1 shRNA
  • Alt name
    Nramp1 shRNA
  • gRNA/shRNA sequence
    unknown
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    55
  • Entrez Gene
    Slc11a1 (a.k.a. Bcg, Ity, Ity1, Lsh, Nramp, Nramp1, ity)
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HpaI (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer mU6-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please see plasmid pLB (addgene #11619) for detailed sequence and map.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLB-915 was a gift from Stephan Kissler (Addgene plasmid # 11620 ; http://n2t.net/addgene:11620 ; RRID:Addgene_11620)

  • For your References section:

    In vivo RNA interference demonstrates a role for Nramp1 in modifying susceptibility to type 1 diabetes. Kissler S, Stern P, Takahashi K, Hunter K, Peterson LB, Wicker LS. Nat Genet. 2006 Apr . 38(4):479-83. 10.1038/ng1766 PubMed 16550170
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