pLB
(Plasmid #11619)

• Purpose: (Empty Backbone) pLB is a modification of pLL3.7 (Addgene#11795). Genetic elements known to prevent epigenetic silencing were added.
• Depositing Lab: Stephan Kissler
• Publication: Kissler et al Nat Genet. 2006 Apr . 38(4):479-83.

Backbone

• Vector backbone: pLL3.7
• Backbone manufacturer: N/A
• Backbone size (bp): 8500
• Vector type: Mammalian Expression, Lentiviral, RNAi, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Promoter: mouse U6
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HpaI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: mU6-F (5'-ATATCCCTTGGAGAAAAGCCTT-3')

Resource Information

• Articles Citing this Plasmid:
  • 26 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pLB is a modification of pLL3.7. Two genetic elements known to prevent epigenetic silencing were added. A fragment of one antirepressor element (#40) was cloned upstream of the mouse U6 promoter and a scaffold-attached region (SAR) was cloned downstream of GFP.

Please see author's map for more detailed information.

Note: A single base pair deletion at position 11 of the mouse U6 promoter in this plasmid does not impair the efficacy of this reagent.
There is also a base pair insertion upstream of the promoter. The depositing lab has no indication that it functionally impairs pLB.