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Addgene

pIND-STAT1-V5
(Plasmid #11618)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11618 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIND
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression ; Ecdysone Inducible
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Signal transducer and activator of transcription 1
  • Alt name
    STAT1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2138
  • Mutation
    no mutations
  • GenBank ID
    BC002704
  • Entrez Gene
    STAT1 (a.k.a. CANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91)
  • Tag / Fusion Protein
    • V5 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Kpn I (not destroyed)
  • 3′ cloning site Sal I (destroyed during cloning)
  • 5′ sequencing primer see pIND
  • 3′ sequencing primer see pIND
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIND-STAT1-V5 was a gift from Steven Johnson (Addgene plasmid # 11618 ; http://n2t.net/addgene:11618 ; RRID:Addgene_11618)
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