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Addgene

pcDNA3-GFP-p48
(Plasmid #11616)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11616 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3-GFP
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Interferon-Stimulated Gene Factor 3
  • Alt name
    ISGF3
  • Alt name
    p48
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1181
  • GenBank ID
    M87503
  • Entrez Gene
    IRF9 (a.k.a. IRF-9, ISGF3, ISGF3G, p48)
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Eco RI (not destroyed)
  • 3′ cloning site Xho I (not destroyed)
  • 5′ sequencing primer na
  • 3′ sequencing primer sp6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-GFP-p48 was a gift from Steven Johnson (Addgene plasmid # 11616 ; http://n2t.net/addgene:11616 ; RRID:Addgene_11616)
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