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Addgene

pcDNA3-HA-NQO1
(Plasmid #11615)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11615 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3-HA
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NAD(P)H dehydrogenase, quinone 1
  • Alt name
    NQO1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1016
  • Mutation
    no mutations
  • GenBank ID
    NM_000903
  • Entrez Gene
    NQO1 (a.k.a. DHQU, DIA4, DTD, NMOR1, NMORI, QR1)
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Eco RI (destroyed during cloning)
  • 3′ cloning site Xho I (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-HA-NQO1 was a gift from Steven Johnson (Addgene plasmid # 11615)
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