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Addgene

pIND-OPN-V5
(Plasmid #11611)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11611 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIND
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression ; Ecdysone Inducible
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Osteopontin
  • Alt name
    OPN
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    902
  • Mutation
    C9L and E160K
  • GenBank ID
    NM_001040060.1 NP_001035149.1
  • Entrez Gene
    SPP1 (a.k.a. BNSP, BSPI, ETA-1, OPN)
  • Tags / Fusion Proteins
    • V5 (C terminal on insert)
    • His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (not destroyed)
  • 3′ cloning site Eco RV (not destroyed)
  • 5′ sequencing primer Ecdysone-Fwd (5'-CTCTGAATACTTTCAACAAGTTAC-3')
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that Addgene's sequencing results identified C9L and E160K mutations in the OPN (SPP1) sequence when compared to GenBank reference sequence NP_001035149.1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIND-OPN-V5 was a gift from Steven Johnson (Addgene plasmid # 11611 ; http://n2t.net/addgene:11611 ; RRID:Addgene_11611)
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