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Addgene

pIRES proHB-EGF NC
(Plasmid #11604)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11604 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRESHyg2
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5800
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HB-EGF
  • Alt name
    proHB-EGF
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    667
  • Mutation
    Non-cleavable HB-EGF: Pro149 to Thr160 replaced with CD4 JM domain.
  • Entrez Gene
    HBEGF (a.k.a. DTR, DTS, DTSF, HEGFL)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NsiI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

JM domain of HB-EGF replaced with JM domain of CD4 (PVENPLYTYDHT replaced with KVLPTWSTPVQP).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIRES proHB-EGF NC was a gift from Rosalyn Adam (Addgene plasmid # 11604 ; http://n2t.net/addgene:11604 ; RRID:Addgene_11604)

  • For your References section:

    Heparin-binding epidermal growth factor-like growth factor stimulates androgen-independent prostate tumor growth and antagonizes androgen receptor function. Adam RM, Kim J, Lin J, Orsola A, Zhuang L, Rice DC, Freeman MR. Endocrinology. 2002 Dec . 143(12):4599-608. 10.1210/en.2002-220561 PubMed 12446587
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