pIRES proHB-EGF CS
(Plasmid
#11603)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 11603 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepIRESHyg2
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 5800
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHB-EGF
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Alt nameproHB-EGF
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Alt namesHB-EGF
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SpeciesH. sapiens (human)
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Insert Size (bp)453
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MutationConstitutively secreted HB-EGF: C-terminal amino acid is Glu151.
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Entrez GeneHBEGF (a.k.a. DTR, DTS, DTSF, HEGFL)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site NsiI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIRES proHB-EGF CS was a gift from Rosalyn Adam (Addgene plasmid # 11603 ; http://n2t.net/addgene:11603 ; RRID:Addgene_11603) -
For your References section:
Heparin-binding epidermal growth factor-like growth factor stimulates androgen-independent prostate tumor growth and antagonizes androgen receptor function. Adam RM, Kim J, Lin J, Orsola A, Zhuang L, Rice DC, Freeman MR. Endocrinology. 2002 Dec . 143(12):4599-608. 10.1210/en.2002-220561 PubMed 12446587