Skip to main content
Addgene

pcDNA3 proHB-EGF CS
(Plasmid #11601)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11601 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcDNA3.1-myc/His(A)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HB-EGF
  • Alt name
    proHB-EGF
  • Alt name
    sHB-EGF
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    453
  • Mutation
    Constitutively secreted HB-EGF: C-terminal amino acid is Glu151.
  • Entrez Gene
    HBEGF (a.k.a. DTR, DTS, DTSF, HEGFL)
  • Tags / Fusion Proteins
    • myc (C terminal on backbone)
    • His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 proHB-EGF CS was a gift from Rosalyn Adam (Addgene plasmid # 11601 ; http://n2t.net/addgene:11601 ; RRID:Addgene_11601)

  • For your References section:

    Heparin-binding epidermal growth factor-like growth factor stimulates androgen-independent prostate tumor growth and antagonizes androgen receptor function. Adam RM, Kim J, Lin J, Orsola A, Zhuang L, Rice DC, Freeman MR. Endocrinology. 2002 Dec . 143(12):4599-608. 10.1210/en.2002-220561 PubMed 12446587
Related items:
Commonly requested with: