pSW725 - pUFO>>
(Plasmid #116000)

• Purpose: destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression
• Depositing Lab: Sebastien Wolf
• Publication: Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.00463. doi: 10.1104/pp.18.00463.

Backbone

• Vector backbone: Z001
• Vector type: Plant Expression
• Selectable markers: Sulfadiazine, mTurquoise

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: Test multiple colonies, see note in Comments section.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pUFO:GR-LhG4:tRBCS:F-H adapter-H-A adapter::pOp6:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 10234
• Tags / Fusion Proteins:
  • SP(ER) (N terminal on insert)
  • HDEL (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pUFO_inZ001_pSW725.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the pOp6 (LacO repeat) region is unstable. Addgene recommends sequencing multiple colonies to isolate full plasmid.