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Addgene

pSW698 - pCLV3>>
(Plasmid #115997)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115997 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    Z001
  • Vector type
    Plant Expression
  • Selectable markers
    Sulfadiazine, mTurquoise

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pCLV3:GR-LhG4:tCLV3:F-H adapter-H-A adapter::pOp6:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    8517
  • Tags / Fusion Proteins
    • SP(ER) (N terminal on insert)
    • HDEL (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that Addgene was able to verify 5 of the 6 LacO repeats. The 6th LacO appears to be present but is difficult to confirm due to the repetitive nature of the sequence. Addgene recommends sequencing this region upon receipt.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSW698 - pCLV3>> was a gift from Sebastien Wolf (Addgene plasmid # 115997)

  • For your References section:

    A Comprehensive Toolkit for Inducible, Cell Type-Specific Gene Expression in Arabidopsis. Schurholz AK, Lopez-Salmeron V, Li Z, Forner J, Wenzl C, Gaillochet C, Augustin S, Vilches-Barro A, Fuchs M, Gebert M, Lohmann JU, Greb T, Wolf S. Plant Physiol. 2018 Jul 19. pii: pp.18.00463. doi: 10.1104/pp.18.00463. 10.1104/pp.18.00463 PubMed 30026289
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